机构：
云南省林业科学院云南省森林植物培育与开发利用重点实验室国家林业局云南珍稀濒特森林植物保护和繁育重点实验室;
中文关键词：
蒜头果;;FAD2;;基因克隆;;功能分析
中文摘要：
FAD2是亚油酸合成的关键酶,是控制高油酸特性的重要基因。本研究采用RT-PCR技术在蒜头果中成功克隆得到一个FAD2基因,命名为MoFAD2,GenBank登录号为MK210593。其含有1 329 bp开放阅读框,编码442个氨基酸。序列比对分析显示MoFAD2拥有FAD2家族特有的3个保守的组氨酸中心位点,蒜头果FAD2与中粒咖啡(Coffea canephora) FAD2的蛋白序列同源性为80.00%。系统进化树分析显示MoFAD2与石榴(Punica granatum)、珙桐(Davidia involucrata)聚为一支。荧光定量PCR分析表明MoFAD2在果实和叶中均有表达,但在蒜头果果实膨大期的表达量最高。研究为将来更好地开发利用蒜头果油用价值和进一步开展FAD2在不饱和脂肪酸生物合成过程中的调控作用研究奠定基础。
英文篇名：
Cloning and Functional Analysis of omega-6 Fatty Acid Desaturase Gene from Malania oleifera
英文作者：
Li Yunqin;Chen Zhonghua;Yuan Xiaolong;Wang Yi;The Key Laboratory of Forestry Plant Cultivation and Utilization, The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration, Yunnan Academy of Forestry;
英文摘要：
omega-6 fatty acid desaturase is the key enzyme in the biosynthesis pathway of linolenic acids. It is an important gene controlling the characteristics of high oleic acid. In this study, aω-6 fatty acid dehydrogenase gene(MoFAD2) was obtained successfully by cloned using RT-PCR technology from Malania oleifera. The gene sequence of MoFAD2 consisted of 1 329 bp open reading frame(ORF) and encoded 442 amino acids, accession number of the MoFAD2 gene in GenBank was MK210593. The proteinic sequence alignment revealed there is 80.00% of similarity between Malania oleifera and Coffea canephora. The phylogenetic tree analysis showed that MoFAD2 and FAD2 from Punica granatum and Davidia involucrata had the same branch, and has 3 domains functionally conserved that is special in the FAD2 family. The fluorescent quantitative reverse PCR certified that the expression of MoFAD2 gene in fruit of the flower withered 3 months is highest. The study laid a foundation for better development and utilization of Malania oleifera fruit oil and further studies on the regulatory role of FAD2 in the biosynthesis of unsaturated fatty acids in the future.
英文关键词：
Malania oleifera;;FAD2;;Gene clone;;Functional analysis
