作者：
丁玉;梁永欣;吴雪花;包婷雯;南杰东智;林鹏程;
机构：
青海民族大学药学院;青海省青藏高原植物化学重点实验室;
中文关键词：
细果角茴香;;指纹图谱;;HPLC;;聚类分析
中文摘要：
目的:建立细果角茴香HPLC指纹图谱评价体系。方法:采用ZORBAX SB-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1mol/L醋酸铵溶液(每1 L溶液中加冰乙酸0.5 m L)为流动相进行梯度洗脱,流速1.0 m L/min,检测波长300 nm,建立了30批细果角茴香的指纹图谱,使用"中药色谱指纹图谱相似度评价系统(2004版A)"软件计算相似度。结果:建立了细果角茴香HPLC指纹图谱共有模式,标定了15个共有峰,相似度在0.867~0.992,用聚类分析对指纹图谱进行模式识别。结论:将指纹图谱和模式识别结合起来对细果角茴香进行质量控制是一种有效的评价方法,为细果角茴香的全面质量控制提供了基础。
英文篇名：
HPLC Fingerprint Analysis for Whole Herb of Tibet Medicine Hypecoum leptocarpum
英文作者：
DING Yu;LIANG Yong-xin;WU Xue-hua;BAO Ting-wen;NAN Jie-dong-zhi;LIN Peng-cheng;College of Pharmacy,Qinghai Nationalities University;Qinghai Provincial Key Laboratory for Chemistry of Plant Resources of Qinghai-Tibet Plateau;
英文摘要：
Objective: To establish the HPLC fingerprint and its evaluation analysis for whole herb of Hypecoum leptocarpum.Methods: ZORBAX SB-C18 column( 250 mm×4. 6 mm,5 μm) was used with acetonitrile-0. 1 mol/L ammonium acetate solution( added 0. 5 m L glacial acetic acid per 1 L) in gradient elution mode. The flow rate was 1. 0 m L/min and the detection wavelength was 300 nm. The fingerprint for the whole herb of Hypecoum leptocarpum from 30 different sources Hypecoum leptocarpum was set up. The similarity assay for whole herb of Hypecoum leptocarpum from 30 different sources was carried out to evaluate their quality by Chinese Material Medical Fingerprint Similarity Evaluation System( 2004 A edition). Results: The common mode of HPLC fingerprint for whole herb of Hypecoum leptocarpum was set up. 15 common peaks in the fingerprint were identified. The similarity of the fingerprint for the whole herb of Hypecoum leptocarpum from 30 different sources was in the range of 0. 867 ~ 0. 992. The cluster analysis was applied to study the HPLC fingerprint and chemical pattern recognition. Conclusion: The combination of fingerprint and chemical pattern recognition is an effective method for the quality control of whole herb of Hypecoum leptocarpum. This study lays the foundation for the full quality evaluation for the whole herb of Hypecoum leptocarpum.
英文关键词：
Hypecoum leptocarpum Hook.f.et Thoms;;Fingerprint;;HPLC;;Cluster analysis
